Enzyme = Catalase
Substrate = Hydrogen Peroxide (H2O2)
Catalase enzyme breaks down the substrate hydrogen peroxide into water and oxygen.
Enzyme Concentration (i.e. How much Catalase is available)
Substrate Concentration (i.e. How much Hydrogen Peroxide is around to break down?)
Ionic Concentration (i.e. How does the concentration of other things in the solution impact the reaction rate?)
Several enzymatic variables will be examined in this lab. You will be using the protein enzyme, catalase. Catalase is found in most cells, even in single-celled eukaryotes like yeast. In this lab, you will extract catalase from a yeast solution and test its catalytic effectiveness on hydrogen peroxide. Catalase speeds up the breakdown of peroxides which may form during respiration (metabolic energy production). This breakdown prevents the peroxide from causing unwanted oxidation of important biomolecules:
2 H2O2 → 2 H2O + O2 (gas)
We will measure enzyme activity by measuring the generation of oxygen gas — a product in the reaction.